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goat anti pdgfr β  (R&D Systems)


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    R&D Systems goat anti pdgfr β
    Goat Anti Pdgfr β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pdgfr%CE%B2/Mouse+PDGF+R+beta+Antibody/pmc13124745-67-41-45
    Average 96 stars, based on 238 article reviews
    goat anti pdgfr β - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination
    Article Snippet: .. Sections were then incubated in blocking solution (10% Donkey serum 0.25% (v/v) TritonTM X-100 in 1X TBS) for 1 h at RT, followed by MOM IgG blocking solution (Vector laboratories) for 1 h at RT before adding the following primary antibodies: rabbit anti-Iba1 (Fujifilm Wako, 019-19741, 1:400), mouse anti-p16 (Abcam, ab54210, 1:500), mouse anti-p21 (Santa Cruz, sc6246, 1:500), goat anti-PDGFRβ (R&D systems, AF1042, 1:200), rat anti-CD3 (eBioscience, 14–0032-85, 1:100), rat anti-B220 (Invitrogen, 14–0452-81, 1:100), rat anti-CD11b (Biorad MCA74G, 1:100), rabbit anti-Olig2 (Millipore Sigma, AB9610, 1:300), rabbit anti-Sox9 (EMD Millipore, AB5535, 1:1500), rabbit anti-RFP (Rockland Immunochemicals 600–401-379, 1:1000) or rabbit anti-DsRd (Clontech, 632496, 1:500), rabbit anti-KI67 (ThermoFischer Scientific, PA5-19462, 1:200), rabbit anti-cleaved-caspase-3 (Cell Signaling, 9661S, 1:100), mouse anti-NF200 (Sigma-Aldrich, N4142, 1:100), and rabbit anti-SMI32 (BioLegend, 801702, 1:500). .. Slides were then washed in TBST and TBS before being incubated for 1 h in the dark at RT with the following fluorescent conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, 1:1000), Alexa Fluor 594 (Invitrogen, 1:500) and Hoechst 33342 (Invitrogen, 1:20,000).

    Article Title: 3D Vessels-on-Chip using isogenic hiPSC-derived VSMCs reveal NOTCH3-driven alterations in brain small vessel disease.
    Article Snippet: .. The slides were incubated at room temperature for 2 h with a primary mouse anti-NOTCH3 antibody (clone 1E4, Millipore) or for 1 h with a primary goat anti-PDGFRβ (AF385, R&D Systems). ..

    Article Title: Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination.
    Article Snippet: .. Sections were then incubated in blocking solution (10% Donkey serum 0.25% (v/v) TritonTM X-100 in 1X TBS) for 1 h at RT, followed by MOM IgG blocking solution (Vector laboratories) for 1 h at RT before adding the following primary antibodies: rabbit anti-Iba1 (Fujifilm Wako, 019-19741, 1:400), mouse anti-p16 (Abcam, ab54210, 1:500), mouse antip21 (Santa Cruz, sc6246, 1:500), goat anti-PDGFRβ (R&D systems, AF1042, 1:200), rat anti-CD3 (eBioscience, 14–0032-85, 1:100), rat anti-B220 (Invitrogen, 14–0452- 81, 1:100), rat anti-CD11b (Biorad MCA74G, 1:100), rabbit anti-Olig2 (Millipore Sigma, AB9610, 1:300), rabbit anti-Sox9 (EMD Millipore, AB5535, 1:1500), rabbit antiRFP (Rockland Immunochemicals 600–401-379, 1:1000) or rabbit anti-DsRd (Clontech, 632496, 1:500), rabbit anti-KI67 (ThermoFischer Scientific, PA5-19462, 1:200), rabbit anti-cleaved-caspase-3 (Cell Signaling, 9661S, 1:100), mouse anti-NF200 (Sigma-Aldrich, N4142, 1:100), and rabbit anti-SMI32 (BioLegend, 801702, 1:500). .. Slides were then washed in TBST and TBS before being incubated for 1 h in the dark at RT with the following fluorescent conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, 1:1000), Alexa Fluor 594 (Invitrogen, 1:500) and Hoechst 33342 (Invitrogen, 1:20,000).

    Article Title: Perichondrium origin of pericyte-septoclast lineage in endochondral bone development.
    Article Snippet: During endochondral ossification of long bones, vascular invasion initiated at the midportion of the cartilaginous templates leads to cartilage matrix resorption with calcified bone collar formation (Long & Ornitz, 2013).. Endothelial cells and pericytes, which constitute the capillaries, cooperate to promote angiogenesis (Payne et al., 2020).. Pericytes envelope the endothelial cells of capillaries and are implicated in vascular morphogenesis and cardiovascular homeostasis (Armulik et al., 2011; DiazFlores et al., 2009).

    Article Title: 3D Vessels-on-Chip using isogenic hiPSC-derived VSMCs reveal NOTCH3-driven alterations in brain small vessel disease
    Article Snippet: .. The slides were incubated at room temperature for 2 h with a primary mouse anti-NOTCH3 antibody (clone 1E4, Millipore) or for 1 h with a primary goat anti-PDGFRβ (AF385, R&D Systems). ..

    Blocking Assay:

    Article Title: Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination
    Article Snippet: .. Sections were then incubated in blocking solution (10% Donkey serum 0.25% (v/v) TritonTM X-100 in 1X TBS) for 1 h at RT, followed by MOM IgG blocking solution (Vector laboratories) for 1 h at RT before adding the following primary antibodies: rabbit anti-Iba1 (Fujifilm Wako, 019-19741, 1:400), mouse anti-p16 (Abcam, ab54210, 1:500), mouse anti-p21 (Santa Cruz, sc6246, 1:500), goat anti-PDGFRβ (R&D systems, AF1042, 1:200), rat anti-CD3 (eBioscience, 14–0032-85, 1:100), rat anti-B220 (Invitrogen, 14–0452-81, 1:100), rat anti-CD11b (Biorad MCA74G, 1:100), rabbit anti-Olig2 (Millipore Sigma, AB9610, 1:300), rabbit anti-Sox9 (EMD Millipore, AB5535, 1:1500), rabbit anti-RFP (Rockland Immunochemicals 600–401-379, 1:1000) or rabbit anti-DsRd (Clontech, 632496, 1:500), rabbit anti-KI67 (ThermoFischer Scientific, PA5-19462, 1:200), rabbit anti-cleaved-caspase-3 (Cell Signaling, 9661S, 1:100), mouse anti-NF200 (Sigma-Aldrich, N4142, 1:100), and rabbit anti-SMI32 (BioLegend, 801702, 1:500). .. Slides were then washed in TBST and TBS before being incubated for 1 h in the dark at RT with the following fluorescent conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, 1:1000), Alexa Fluor 594 (Invitrogen, 1:500) and Hoechst 33342 (Invitrogen, 1:20,000).

    Article Title: Senescent cell reduction does not improve recovery in mice under experimental autoimmune encephalomyelitis (EAE) induced demyelination.
    Article Snippet: .. Sections were then incubated in blocking solution (10% Donkey serum 0.25% (v/v) TritonTM X-100 in 1X TBS) for 1 h at RT, followed by MOM IgG blocking solution (Vector laboratories) for 1 h at RT before adding the following primary antibodies: rabbit anti-Iba1 (Fujifilm Wako, 019-19741, 1:400), mouse anti-p16 (Abcam, ab54210, 1:500), mouse antip21 (Santa Cruz, sc6246, 1:500), goat anti-PDGFRβ (R&D systems, AF1042, 1:200), rat anti-CD3 (eBioscience, 14–0032-85, 1:100), rat anti-B220 (Invitrogen, 14–0452- 81, 1:100), rat anti-CD11b (Biorad MCA74G, 1:100), rabbit anti-Olig2 (Millipore Sigma, AB9610, 1:300), rabbit anti-Sox9 (EMD Millipore, AB5535, 1:1500), rabbit antiRFP (Rockland Immunochemicals 600–401-379, 1:1000) or rabbit anti-DsRd (Clontech, 632496, 1:500), rabbit anti-KI67 (ThermoFischer Scientific, PA5-19462, 1:200), rabbit anti-cleaved-caspase-3 (Cell Signaling, 9661S, 1:100), mouse anti-NF200 (Sigma-Aldrich, N4142, 1:100), and rabbit anti-SMI32 (BioLegend, 801702, 1:500). .. Slides were then washed in TBST and TBS before being incubated for 1 h in the dark at RT with the following fluorescent conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, 1:1000), Alexa Fluor 594 (Invitrogen, 1:500) and Hoechst 33342 (Invitrogen, 1:20,000).

    other:

    Article Title: SYK-dependent lipid handling in monocyte-derived macrophages governs functional recovery after spinal cord injury.
    Article Snippet: The corresponding secondary antibodies used were as follows: Alexa Fluor 488 (1:500, A-21208, A-21206, A-11055, Invitrogen, USA) and Alexa Fluor 555 (1:500, A-31572, A-48270, A21432, Invitrogen, USA).

    Article Title: Endothelial delivery of simvastatin by LRP1-targeted nanoparticles ameliorates pathogenesis of alzheimer's disease in a mouse model.
    Article Snippet: Rabbit anti-LRP1 (ab92544, Abcam), Rat anti-CD31 (550274, BD), Mouse anti-NeuN (94403, CST), Rat anti-NeuN (ab279297, Abcam), Rabbit-anti-GFAP (3670T, CST), Goat-anti-IBA-1 (AB5076, Abcam), Goat anti-PDGFRβ (AF385, R&D systems), Mouse antiAβ-6E10 (803015, biolegend), Rabbit anti-synaptophysin (SYN) (ab32127, Abcam), Rabbit anti-ZO1 (ab276131, Abcam), Rabbit anti-Occludin (ab216327, Abcam), Rabbit anti-Claudin 5 (AF5216, AffinitY), Rabbit antiRAGE (ab216329, Abcam), Mouse anti-APP (MAB348, Merke), Mouse anti-BACE1 (NBP2-37261, Novus), Mouse anti-GAPDH (FD0063-100, FD), β-actin(66009-1, Proteintech), Alexa Fluor 555 Donkey anti-Rabbit (A32794, Invitrogen), Alexa Fluor 488 Donkey anti Rabbit (A21206, Invitrogen), Alexa Fluor 555 Donkey anti Mouse (A32773, Invitrogen), Alexa Fluor 488 Donkey anti Mouse (A32766, Invitrogen), Alexa Fluor 488 Donkey anti Goat (A11055, Invitrogen), Alexa Fluor 488 Donkey anti-Rat (A21208, Invitrogen), and HRP AffiniPure Goat anti-Mouse IgG (H + L) (FD0142, FD), HRP AffiniPure Goat anti-Rabbit IgG (H + L) (FD0128, FD) were used.

    Saline:

    Article Title: Perichondrium origin of pericyte-septoclast lineage in endochondral bone development.
    Article Snippet: During endochondral ossification of long bones, vascular invasion initiated at the midportion of the cartilaginous templates leads to cartilage matrix resorption with calcified bone collar formation (Long & Ornitz, 2013).. Endothelial cells and pericytes, which constitute the capillaries, cooperate to promote angiogenesis (Payne et al., 2020).. Pericytes envelope the endothelial cells of capillaries and are implicated in vascular morphogenesis and cardiovascular homeostasis (Armulik et al., 2011; DiazFlores et al., 2009).

    Immunostaining:

    Article Title: A neurovascular template guides the spatial and functional compartmentalization of the adrenal gland
    Article Snippet: Slides were mounted with Fluorescent Mounting Medium (Dako S3023). .. Primary antibodies used for immunostaining: rabbit anti-Caspase3 (1:300; BD Pharm 559565); rabbit anti-CD206 (1:500; Abcam 64693); rat anti-CD31 (1:200; BD Biosciences 550274); goat anti-CD31 (1:1000; R&D systems AF3628); rat anti-CD86 (1:100; Biolegend 105008); rabbit anti-ChromograninA (1:500; Abcam ab254322); rabbit anti-Claudin5 (1:300 Invitrogen 34-1600); rat anti-F4-80 (1:300 Abcam ab6640); rabbit anti-GFP (1:5000; Thermo Fisher Scientific A6455); chicken anti-GFP (1:1000; Abcam ab13970); rabbit anti-IBA1 (1:500 Wako 019-19741); rabbit anti-nNOS (1:1000; Immustar 24287); goat anti-PDGFRβ (1:200; R&D systems AF1042); goat anti-PHOX2B (1:500; R&D systems AF4940); PLVAP (1:200 BD Bioscience 553849); rabbit anti-RFP (1:1000 MBL Life Science PM005);rabbit anti-SF1 (1:400; Proteintech 18658-1-AP); rabbit anti-SOX10 (1:1000; Abcam ab155279); rabbit anti-TH (1:1000; Millipore AB152); chicken anti-TH (1:500; Abcam ab76442); goat anti-Tie2 (1:200; R&D systems AF762-SP); rabbit anti-TRKA (); rat anti-vCAM1 (1:100 BD Pharm 550547); rabbit anti-VaChat (1:1000; Synaptic Systems 139103); rat anti-VEGFR2 (1:100; BD Pharm 550549). .. Secondary antibodies used for immunostaining: goat or donkey anti-goat/rabbit/rat Alexa Fluor 488-, 555-, 647-conjugated (1:1000; Thermo Fisher Scientific); donkey anti-chick Alexa Fluor-488 (1:500; JacksonImmunoResearch 703-545-155); donkey anti-rat Cy3 (1:500; JacksonImmunoResearch 712-165-153); donkey anti-rabbit Alexa Fluor-405 (1:250; JacksonImmunoResearch).



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    Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + <t>(PDGFR-β</t> + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file
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    Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + <t>(PDGFR-β</t> + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file
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    Image Search Results


    Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + (PDGFR-β + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Journal: Bone Research

    Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

    doi: 10.1038/s41413-026-00507-7

    Figure Lengend Snippet: Conditional knockout of Tgfβ1 in macrophage lineage cells reduces pSmad2 levels and promotes functional recovery in SCI mice. a – d ELISA analysis showing the concentration of TGF-β in the spinal cord and the serum after SCI in each mouse for the groups indicated (* P < 0.05, ** P < 0.01, n = 4). e Representative Western blots showing the activation of phosphorylated Smad (pSmad) signaling. f Representative images of immunofluorescent staining of pSmad2 + (red), platelet-derived growth factor receptor-β + (PDGFR-β + ) (green) pericytes , and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. g Representative immunofluorescence images of PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 2 weeks after SCI. Scale bars, 200 µm. h Quantitative analysis of the number of pSmad2 + cells (**** P < 0.000 1, n = 6). i Quantitative analysis of the number of PDGFR-β + cells (** P < 0.01, n = 6). j , k Illustrations of the hot plate test and the von Frey test. l Quantitative analysis of hindpaw withdrawal time responding to temperature (hot plate test, ** P < 0.01, n = 8). m , n Quantitative analysis of hindpaw withdrawal frequency responding to mechanical stimulation (von Frey test, 0.7 mN and 3.9 mN, * P < 0.05, ** P < 0.01, n = 8). o Quantitative analysis of Basso Mouse Scale (BMS) score between Tgfb1 flox / flox control mice after SCI, Tgfb1 LysM-cre −/− mice after SCI, and Tgfb1 flox/flox control mice without SCI (* P < 0.05, n = 8). PWF paw withdrawal frequency. LF left forepaw, RF right forepaw, RH right hindpaw. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Article Snippet: The sections were incubated with primary antibodies to rabbit 5-HT (1:50, sc-65495, Santa Cruz Biotechnology, Dallas, TX, USA), mouse β-III-tubulin (1:100, MA1-118, Invitrogen, Carlsbad, CA, USA), rabbit PGP9.5 (1:250, ab108986, Abcam, Cambridge, UK), mouse PGP9.5 (1:50, ab8189, Abcam), rabbit Fibronectin (1:100, ab2413, Abcam), mouse Fibronectin (1:100, ab6328, Abcam), rabbit Collagen III (1:100, ab7778, Abcam), chicken GFAP (1:500, ab4674, Abcam), mouse Collagen1α1 (1:50, sc-293182, Santa Cruz Biotechnology), rabbit Phospho SAMD2 (1:100, 44-244 G, Invitrogen), rabbit PDGFR-β (1:100, ab32570, Abcam), goat PDGFR-β (1:100, AF1042, R&D Systems), rabbit FSP1 (1:300, 07-2274, MilliporeSigma, Burlington, MA, USA), and chicken green fluorescent protein (1:250, ab13970, Abcam) overnight at 4 °C.

    Techniques: Knock-Out, Functional Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Activation Assay, Staining, Derivative Assay, Immunofluorescence, Hot Plate Test, Control

    Conditional knockout of TGF-β type 2 receptor ( Tgfbr2 ) in pericytes reduces fibrotic scar formation in SCI mice. a Schematic diagram of Tgfbr2 knockout in Glast-Cre + pericytes. b Representative images of immunofluorescent analysis of PGP9.5 + (red) nerve fibers, fibronectin + (green) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. c , d Quantitative analysis of the intensity value of PGP9.5 and fibronectin (* P < 0.05, n = 6). e Representative images of immunofluorescent analysis of nerve axon–specific β-III-tubulin + (green), collagen III + (red) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. Right images are high resolution versions of the boxed regions in the left images. Scale bar, 50 µm. f Quantitative analysis of the intensity value of collagen III (* P < 0.05, n = 6). g Representative images of immunofluorescent analysis of neurotransmitter marker 5-HT + (red), nerve axon–specific β-III-tubulin + (green), and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in Tgfbr2 flox/flox sham group mice, Tgfbr2 flox/flox control mice, and Tgfbr2 Glast-creER −/− mice at 4 weeks after SCI. Scale bar, 200 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. h , i Quantitative analysis of the intensity value of 5-HT and β-III-tubulin (* P < 0.05, n = 6). j Representative images of immunofluorescent analysis of pSmad2 + (red), PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. k , l Quantitative analysis of the intensity mean value of PDGFR-β and the number of pSmad2 + cells (*** P < 0.001, **** P < 0.000 1, n = 6). m Representative Western blots showing the activation of pSmad signaling. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Journal: Bone Research

    Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

    doi: 10.1038/s41413-026-00507-7

    Figure Lengend Snippet: Conditional knockout of TGF-β type 2 receptor ( Tgfbr2 ) in pericytes reduces fibrotic scar formation in SCI mice. a Schematic diagram of Tgfbr2 knockout in Glast-Cre + pericytes. b Representative images of immunofluorescent analysis of PGP9.5 + (red) nerve fibers, fibronectin + (green) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. c , d Quantitative analysis of the intensity value of PGP9.5 and fibronectin (* P < 0.05, n = 6). e Representative images of immunofluorescent analysis of nerve axon–specific β-III-tubulin + (green), collagen III + (red) fibrotic scar, and DAPI (blue) staining of nuclei at 4 weeks after SCI. Scale bar, 200 µm. Right images are high resolution versions of the boxed regions in the left images. Scale bar, 50 µm. f Quantitative analysis of the intensity value of collagen III (* P < 0.05, n = 6). g Representative images of immunofluorescent analysis of neurotransmitter marker 5-HT + (red), nerve axon–specific β-III-tubulin + (green), and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in Tgfbr2 flox/flox sham group mice, Tgfbr2 flox/flox control mice, and Tgfbr2 Glast-creER −/− mice at 4 weeks after SCI. Scale bar, 200 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. h , i Quantitative analysis of the intensity value of 5-HT and β-III-tubulin (* P < 0.05, n = 6). j Representative images of immunofluorescent analysis of pSmad2 + (red), PDGFR-β + (green) pericytes and DAPI (blue) staining of nuclei at 7 days after SCI. Scale bar, 10 µm. k , l Quantitative analysis of the intensity mean value of PDGFR-β and the number of pSmad2 + cells (*** P < 0.001, **** P < 0.000 1, n = 6). m Representative Western blots showing the activation of pSmad signaling. Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Article Snippet: The sections were incubated with primary antibodies to rabbit 5-HT (1:50, sc-65495, Santa Cruz Biotechnology, Dallas, TX, USA), mouse β-III-tubulin (1:100, MA1-118, Invitrogen, Carlsbad, CA, USA), rabbit PGP9.5 (1:250, ab108986, Abcam, Cambridge, UK), mouse PGP9.5 (1:50, ab8189, Abcam), rabbit Fibronectin (1:100, ab2413, Abcam), mouse Fibronectin (1:100, ab6328, Abcam), rabbit Collagen III (1:100, ab7778, Abcam), chicken GFAP (1:500, ab4674, Abcam), mouse Collagen1α1 (1:50, sc-293182, Santa Cruz Biotechnology), rabbit Phospho SAMD2 (1:100, 44-244 G, Invitrogen), rabbit PDGFR-β (1:100, ab32570, Abcam), goat PDGFR-β (1:100, AF1042, R&D Systems), rabbit FSP1 (1:300, 07-2274, MilliporeSigma, Burlington, MA, USA), and chicken green fluorescent protein (1:250, ab13970, Abcam) overnight at 4 °C.

    Techniques: Knock-Out, Staining, Marker, Control, Western Blot, Activation Assay

    LepR + MSCs are the primary cells for fibrotic scar formation. a , b Diagram illustrating the genetic strategy to trace type A pericytes and the timeline of tamoxifen injection. c Representative images of immunofluorescent analysis of tdT + (red) type A pericytes, PDGFR-β + (green) pericytes, and DAPI (blue) staining of nuclei in the spinal cord lesion site of T10 wild-type (WT) mice at 2 weeks after SCI. Scale bar, 50 µm. Left images are high-resolution versions of the boxed regions in the right images. Scale bar, 10 µm. d Quantitative analysis of percentage of PDGFR-β cells/tdTomato + type A pericytes cells (*** P < 0.001, n = 4). e Representative images of immunofluorescent analysis of collagen III + (Col-III green) fibrotic scar, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in 13C4 group control mice and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. f Quantitative analysis of percentage of collagen III + area /tdTomato + type A pericytes field (*** P < 0.001, n = 4). g Representative images of immunofluorescent analysis of LepR + (green) MSCs, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in sham group mice, 13C4 group control mice, and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. h Quantitative analysis of the percentage of LepR + cells/tdTomato + cells (* P < 0.05, n = 4). i Representative images of immunofluorescent analysis of LepR + (green) MSCs, collagen III + (Col-III, green) fibrotic scar , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. j Quantitative analysis of the percentage of collagen III + area/ LepR + MSCs field (** P < 0.01, n = 4). k Representative images of immunofluorescent analysis of LepR + (green) MSCs, FSP1 + (red) type A pericytes , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. l Quantitative analysis of the percentage of LepR + MSCs/FSP1 + cells (** P < 0.01, n = 4). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Journal: Bone Research

    Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

    doi: 10.1038/s41413-026-00507-7

    Figure Lengend Snippet: LepR + MSCs are the primary cells for fibrotic scar formation. a , b Diagram illustrating the genetic strategy to trace type A pericytes and the timeline of tamoxifen injection. c Representative images of immunofluorescent analysis of tdT + (red) type A pericytes, PDGFR-β + (green) pericytes, and DAPI (blue) staining of nuclei in the spinal cord lesion site of T10 wild-type (WT) mice at 2 weeks after SCI. Scale bar, 50 µm. Left images are high-resolution versions of the boxed regions in the right images. Scale bar, 10 µm. d Quantitative analysis of percentage of PDGFR-β cells/tdTomato + type A pericytes cells (*** P < 0.001, n = 4). e Representative images of immunofluorescent analysis of collagen III + (Col-III green) fibrotic scar, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in spinal cord lesion site of T10 in 13C4 group control mice and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. f Quantitative analysis of percentage of collagen III + area /tdTomato + type A pericytes field (*** P < 0.001, n = 4). g Representative images of immunofluorescent analysis of LepR + (green) MSCs, tdT + (red) type A pericytes, and DAPI (blue) staining of nuclei in sham group mice, 13C4 group control mice, and 1D11 group mice at 4 weeks after SCI. Scale bar, 50 µm. h Quantitative analysis of the percentage of LepR + cells/tdTomato + cells (* P < 0.05, n = 4). i Representative images of immunofluorescent analysis of LepR + (green) MSCs, collagen III + (Col-III, green) fibrotic scar , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. j Quantitative analysis of the percentage of collagen III + area/ LepR + MSCs field (** P < 0.01, n = 4). k Representative images of immunofluorescent analysis of LepR + (green) MSCs, FSP1 + (red) type A pericytes , and DAPI (blue) staining of nuclei. Scale bar, 50 µm. l Quantitative analysis of the percentage of LepR + MSCs/FSP1 + cells (** P < 0.01, n = 4). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Article Snippet: The sections were incubated with primary antibodies to rabbit 5-HT (1:50, sc-65495, Santa Cruz Biotechnology, Dallas, TX, USA), mouse β-III-tubulin (1:100, MA1-118, Invitrogen, Carlsbad, CA, USA), rabbit PGP9.5 (1:250, ab108986, Abcam, Cambridge, UK), mouse PGP9.5 (1:50, ab8189, Abcam), rabbit Fibronectin (1:100, ab2413, Abcam), mouse Fibronectin (1:100, ab6328, Abcam), rabbit Collagen III (1:100, ab7778, Abcam), chicken GFAP (1:500, ab4674, Abcam), mouse Collagen1α1 (1:50, sc-293182, Santa Cruz Biotechnology), rabbit Phospho SAMD2 (1:100, 44-244 G, Invitrogen), rabbit PDGFR-β (1:100, ab32570, Abcam), goat PDGFR-β (1:100, AF1042, R&D Systems), rabbit FSP1 (1:300, 07-2274, MilliporeSigma, Burlington, MA, USA), and chicken green fluorescent protein (1:250, ab13970, Abcam) overnight at 4 °C.

    Techniques: Injection, Staining, Control

    Neonatal mice completely recover from SCI without scarring. a Schematic diagram illustrating the timeline of the experimental procedures. b Representative images of immunofluorescent analysis of pSmad2 (red), PDGFR-β (green), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2, 7, and 12 with or without SCI. Scale bar, 50 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. c , d Quantitative analysis of the number of pSmad2 + cells and PDGFR-β + cells (** P < 0.01, *** P < 0.001, **** P < 0.000 1, n = 6). e Representative images of immunofluorescent analysis of β-III-tubulin (green), collagen III (red), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2 and 12, and adult mice with or without SCI. Scale bar, 200 µm. f , g Quantitative analysis of the intensity mean value of collagen III + fibrotic scar and β-III-tubulin + nerves (* P < 0.05, **** P < 0.000 1, n = 6). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Journal: Bone Research

    Article Title: TGF-β-induced fibrotic scar formation limits recovery of spinal cord injury

    doi: 10.1038/s41413-026-00507-7

    Figure Lengend Snippet: Neonatal mice completely recover from SCI without scarring. a Schematic diagram illustrating the timeline of the experimental procedures. b Representative images of immunofluorescent analysis of pSmad2 (red), PDGFR-β (green), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2, 7, and 12 with or without SCI. Scale bar, 50 µm. Right images are high-resolution versions of the boxed regions in the left images. Scale bar, 50 µm. c , d Quantitative analysis of the number of pSmad2 + cells and PDGFR-β + cells (** P < 0.01, *** P < 0.001, **** P < 0.000 1, n = 6). e Representative images of immunofluorescent analysis of β-III-tubulin (green), collagen III (red), and DAPI (blue) in the spinal cord lesion site in neonatal mice on days 2 and 12, and adult mice with or without SCI. Scale bar, 200 µm. f , g Quantitative analysis of the intensity mean value of collagen III + fibrotic scar and β-III-tubulin + nerves (* P < 0.05, **** P < 0.000 1, n = 6). Statistical significance was determined by multifactorial ANOVA, and all data are shown as means ± standard deviations. Source data are provided as a Source Data file

    Article Snippet: The sections were incubated with primary antibodies to rabbit 5-HT (1:50, sc-65495, Santa Cruz Biotechnology, Dallas, TX, USA), mouse β-III-tubulin (1:100, MA1-118, Invitrogen, Carlsbad, CA, USA), rabbit PGP9.5 (1:250, ab108986, Abcam, Cambridge, UK), mouse PGP9.5 (1:50, ab8189, Abcam), rabbit Fibronectin (1:100, ab2413, Abcam), mouse Fibronectin (1:100, ab6328, Abcam), rabbit Collagen III (1:100, ab7778, Abcam), chicken GFAP (1:500, ab4674, Abcam), mouse Collagen1α1 (1:50, sc-293182, Santa Cruz Biotechnology), rabbit Phospho SAMD2 (1:100, 44-244 G, Invitrogen), rabbit PDGFR-β (1:100, ab32570, Abcam), goat PDGFR-β (1:100, AF1042, R&D Systems), rabbit FSP1 (1:300, 07-2274, MilliporeSigma, Burlington, MA, USA), and chicken green fluorescent protein (1:250, ab13970, Abcam) overnight at 4 °C.

    Techniques: